ROTI®Garose-His/Ni HPBeads, for biochemistry, 100 ml, plastic

For the isolation of very high yields of pure His-tagged proteins under non-reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.

ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.

• Superior recovery rate of pure His-tagged proteins
• Very low Nickel leaching
• Compatible with denaturing reagents

The matrix of ROTI®Garose His/Ni products consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. ROTI®Garose His/Ni beads give very high yields of pure His-tagged protein with considerably low metal contamination.

Directions for use

The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.

The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).

A special feature is the modified polychelate linker used in the ROTI®Garose-His/Ni HPBeads plus (0806). For more detailed data please see this product.

For insulation under reducing conditions we recommend ROTI®Garose-His/Ni HPBeads plus or ROTI®Garose-His/Ni NTA beads.