Ultrafree®-DA gel extraction kit, MILLIPORE, 50 unit(s)

for molecular biology

For easy and speedy extraction of DNA fragments from agarose gels.

• Extraction in 10 minutes
• High recovery of fully intact DNA
• Highly efficient with all gel running buffers
• Easy application - even during student courses
• Convenient handling even of large sample numbers

Recovery of functionally intact DNA in one spin-step. The agarose slice is placed directly into the pre-assembled device and centrifuged for 10 minutes (fig. B). Melting or enzymatic digestion is not required. The agarose slice is nebulised and the DNA is filtered and purified during centrifugation. The eluate may simply be stored away in the extraction tube or collected from the tube in order to apply the DNA to subsequent analyses.

Directions for use

Compatible with all agaroses with standard gelling temperature (not compatible with Low-Melting agarose). Suitable for fragments of 100 bp up to 10,000 bp. Ideal also for labelled DNA, e.g. after fluorescence- or radioactive labelling. Reliable recovery rates for fragments of 100 bp up to 2,000 bp.

The gel extraction units may be used with all standard gel running buffers. Following elution from gels run with ROTIPHORESE® 10x TAE buffer light (Art. No. 0122.1), the DNA may be applied directly to cloning or sequencing.

Compatible with all standard Mini-centrifuges.