Immobilon-P, pore size 0,45 µm, roll, 375 x 30 cm, PVDF-membrane, 1 roll(s)

Original PVDF membrane from Millipore, for outstanding Western Blots.

The Immobilon-P membrane consists of microporous polyvinylidene fluoride (PVDF) with a high binding capacity for all biomolecules, particularly for proteins, independently of the transfer method or gel used. The efficient signal-to-noise ratio allows long exposure times and provides a high sensitivity during detection even when working with low protein amounts.

Directions for use

Compared to nitrocellulose the Immobilon-P membrane is much easier to handle and much better to stain, and shows superior resistance to organic solvents. Furthermore, the open pore structure provides easy access of antibodies or other peptides used for detection to bound protein, and enables efficient removal of unbound molecules, efficiently reducing the background.

Immobilon-PVDF membrane is ideal when performing immunodetection. The rapid immunodetection method additionally provides a tool to reduce the time span needed for the process by several hours. During Western Blot, the superior qualities of the PVDF membrane not only supersede a blocking step, but also reduce number and time span of washing steps considerably without compromising on specificity or sensitivity.

Application examples

Western-blot, Dot- and Slot-blot, amino acid analysis, binding assays, detection of glycoproteins, N-terminal peptide sequencing.

Detection systems: radioactivity, chemoluminescence, colour