ROTI®Load DNAstain 3 Green,, 6x DNA gel loading buffer, ready-to-use, 1.8 ml

Loading bufer with the fluorescent dye BrightGreen for staining of double stranded nucleic acid in agarose and polyacrylamide gels.

• Alternative for ethidium bromide, non-toxic, non-mutagen
• 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band
• For use with common broad-band ethidium bromide photo filters
• Excitation possible via UV light (254 nm) and blue light (495 nm)
• Compatible with all usual down-stream applications.

ROTI®Load DNAstain Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye BrightGreen detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by BrightGreen; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.

BrightGreen bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1).

When excited, nucleic acid-bound BrightGreen emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.

The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI®Load DNAstain Green.

Wavelength

Excitation maximum (bound to DNA): 254 nm and 495 nm

Emission maximum (bound to DNA): 521 nm