3'-Azido-2'3'-ddATP, ≥95 %, 10 µl, plastic

This azide modified nucleotide closely resembles a natural nucleotide and enables the possibility of chemoenzymatic labeling to mRNA or ssDNA. Due to the small size of the azide group, 3'-Azido-2',3'-ddATP is well accepted by T7 RNA polymerase, poly(A) polymerase or terminale deoxynucleotidyl transferase (Tdt) for enzymatic incorporation. This is highly beneficial, as labeling of mRNA or ssDNA with our approach is modular and not limited to enzymatic compatibility of bulky pre-labeled nucleotides. Moreover, only a very limited number of such pre-modified nucleotides, mainly dye-labeled ones, are commercially available. Internal and external studies successfully showed e.g. that the poly(A) polymerase adds specifically and sequence independently single azido terminator to the 3'-end of RNA. The main advantage: Any mRNA or ssDNA can be site-specifically labeled without special requirements or altered production protocols at the 3'-end