Poly(A)-ClickSeq Library Prep Kit 12 R., 1 kit, cardboard

Poly(A)-ClickSeq (PAC-Seq) is a library preparation method for NGS that specifically targets the 3′ ends of polyadenylated RNA, such as eukaryotic mRNAs. Unlike conventional RNA-seq which covers entire transcripts, PAC-Seq provides a cost-effective and streamlined approach for differential gene expression analysis and poly(A) site mapping, making it ideal for studies of alternative polyadenylation.The method uses 3′-azido-nucleotides (AzVTPs) in the reverse transcription (RT) step. RT is primed from the poly(A) tail using an unanchored oligo-dT primer, and only AzATP, AzGTP, and AzCTP are used—causing termination upstream of the poly(A) junction in the 3′UTR. The resulting azido-blocked cDNA fragments are then click-ligated to alkyne-functionalized adapters and PCR-amplified to generate sequencing-ready libraries.